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monoclonal antibody sars-cov-1/sars-cov-2 spike protein s2 (1a9)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher monoclonal antibody sars-cov-1/sars-cov-2 spike protein s2 (1a9)
    Monoclonal Antibody Sars Cov 1/Sars Cov 2 Spike Protein S2 (1a9), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spike+protein+s2+(1a9)/ion+ampliseq+sars+cov+2+research+panel/pm36254158-284-1-15
    Average 90 stars, based on 1 article reviews
    monoclonal antibody sars-cov-1/sars-cov-2 spike protein s2 (1a9) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Measuring Concentration of Nanoparticles in Polydisperse Mixtures Using Interferometric Nanoparticle Tracking Analysis.
    Article Snippet: .. For immunofluorescence measurements, 100 μL of inactivated SARS-CoV-2 samples were incubated 90 min at room temperature in glass-bottom dishes (ibidi), unbound particles removed, samples blocked with 4% BSA (w/v) in PBS and incubated overnight at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech) diluted 1:1000. .. Samples were washed 3 times for 5 min with PBS and incubated for 2 h at room temperature with secondary antibodies antimouse AF488 and antirabbit AF561 (Thermo Fisher) diluted 1:1000.

    Article Title: Neurons cytoskeletal architecture remodeling during the replication cycle of mouse coronavirus MHV-JHM: a morphological in vitro study
    Article Snippet: Likewise, microtubules were stained for 60 min in a wet chamber with indirect immunofluorescence with Anti-β-Tubulin III antibody produced in rabbit (dilution 1:200, Sigma Aldrich, Darmstadt, Germany) and then visualized with a secondary antibody, Texas Red goat anti-rabbit IgG (dilution 1:2000, Sigma- Aldrich, Darmstadt, Germany) for 60 min. .. The presence of viral antigen was determined by indirect immunofluorescence, using SARS-CoV/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9) (ThermoFisher, Waltham MA, USA, dilution 1:250) incubated overnight at 4°C. ..

    Article Title: Neurons cytoskeletal architecture remodeling during the replication cycle of mouse coronavirus MHV-JHM: a morphological in vitro study.
    Article Snippet: Likewise, microtubules were stained for 60 min in a wet chamber with indirect immunofluorescence with Anti-β-Tubulin III antibody produced in rabbit (dilution 1:200, Sigma Aldrich, Darmstadt, Germany) and then visualized with a secondary antibody, Texas Red goat anti-rabbit IgG (dilution 1:2000, Sigma- Aldrich, Darmstadt, Germany) for 60 min. .. The presence of viral antigen was determined by indirect immunofluorescence, using SARS-CoV/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9) (ThermoFisher, Waltham MA, USA, dilution 1:250) incubated overnight at 4°C. ..

    Article Title: Measuring Concentration of Nanoparticles in Polydisperse Mixtures Using Interferometric Nanoparticle Tracking Analysis
    Article Snippet: .. For immunofluorescence measurements, 100 μL of inactivated SARS-CoV-2 samples were incubated 90 min at room temperature in glass-bottom dishes (ibidi), unbound particles removed, samples blocked with 4% BSA (w/v) in PBS and incubated overnight at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech) diluted 1:1000. .. Samples were washed 3 times for 5 min with PBS and incubated for 2 h at room temperature with secondary antibodies antimouse AF488 and antirabbit AF561 (Thermo Fisher) diluted 1:1000.

    Article Title: Measuring concentration of nanoparticles in polydisperse mixtures using interferometric nanoparticle tracking analysis (iNTA)
    Article Snippet: .. For immunofluorescence measurements, 100 μl of inactivated SARS-CoV-2 samples were incubated 90 min at room temperature in glass-bottom dishes (ibidi), unbound particles removed, samples blocked with 4 % BSA (w/v) in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech) diluted 1:1000. .. Samples were washed 3 times for 5 minutes with PBS and incubated for 2 hours at room temperature with secondary antibodies anti-mouse AF488 and anti-rabbit AF561 (Thermo Fisher) diluted 1:1000.

    Incubation:

    Article Title: Measuring Concentration of Nanoparticles in Polydisperse Mixtures Using Interferometric Nanoparticle Tracking Analysis.
    Article Snippet: .. For immunofluorescence measurements, 100 μL of inactivated SARS-CoV-2 samples were incubated 90 min at room temperature in glass-bottom dishes (ibidi), unbound particles removed, samples blocked with 4% BSA (w/v) in PBS and incubated overnight at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech) diluted 1:1000. .. Samples were washed 3 times for 5 min with PBS and incubated for 2 h at room temperature with secondary antibodies antimouse AF488 and antirabbit AF561 (Thermo Fisher) diluted 1:1000.

    Article Title: Neurons cytoskeletal architecture remodeling during the replication cycle of mouse coronavirus MHV-JHM: a morphological in vitro study
    Article Snippet: Likewise, microtubules were stained for 60 min in a wet chamber with indirect immunofluorescence with Anti-β-Tubulin III antibody produced in rabbit (dilution 1:200, Sigma Aldrich, Darmstadt, Germany) and then visualized with a secondary antibody, Texas Red goat anti-rabbit IgG (dilution 1:2000, Sigma- Aldrich, Darmstadt, Germany) for 60 min. .. The presence of viral antigen was determined by indirect immunofluorescence, using SARS-CoV/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9) (ThermoFisher, Waltham MA, USA, dilution 1:250) incubated overnight at 4°C. ..

    Article Title: Neurons cytoskeletal architecture remodeling during the replication cycle of mouse coronavirus MHV-JHM: a morphological in vitro study.
    Article Snippet: Likewise, microtubules were stained for 60 min in a wet chamber with indirect immunofluorescence with Anti-β-Tubulin III antibody produced in rabbit (dilution 1:200, Sigma Aldrich, Darmstadt, Germany) and then visualized with a secondary antibody, Texas Red goat anti-rabbit IgG (dilution 1:2000, Sigma- Aldrich, Darmstadt, Germany) for 60 min. .. The presence of viral antigen was determined by indirect immunofluorescence, using SARS-CoV/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9) (ThermoFisher, Waltham MA, USA, dilution 1:250) incubated overnight at 4°C. ..

    Article Title: Measuring Concentration of Nanoparticles in Polydisperse Mixtures Using Interferometric Nanoparticle Tracking Analysis
    Article Snippet: .. For immunofluorescence measurements, 100 μL of inactivated SARS-CoV-2 samples were incubated 90 min at room temperature in glass-bottom dishes (ibidi), unbound particles removed, samples blocked with 4% BSA (w/v) in PBS and incubated overnight at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech) diluted 1:1000. .. Samples were washed 3 times for 5 min with PBS and incubated for 2 h at room temperature with secondary antibodies antimouse AF488 and antirabbit AF561 (Thermo Fisher) diluted 1:1000.

    Article Title: Measuring concentration of nanoparticles in polydisperse mixtures using interferometric nanoparticle tracking analysis (iNTA)
    Article Snippet: .. For immunofluorescence measurements, 100 μl of inactivated SARS-CoV-2 samples were incubated 90 min at room temperature in glass-bottom dishes (ibidi), unbound particles removed, samples blocked with 4 % BSA (w/v) in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech) diluted 1:1000. .. Samples were washed 3 times for 5 minutes with PBS and incubated for 2 hours at room temperature with secondary antibodies anti-mouse AF488 and anti-rabbit AF561 (Thermo Fisher) diluted 1:1000.

    other:

    Article Title: Identification and differential usage of a host metalloproteinase entry pathway by SARS-CoV-2 Delta and Omicron.
    Article Snippet: Spike Protein S2 (1A9) ThermoFisher Scientific Cat#MA5-35946 RRID: AB_2866558

    Article Title: Hybrid alphavirus-SARS-CoV-2 particle and methodology of making and using same
    Article Snippet: Virion lysates were analyzed by SDS-PAGE and western blot using Spike Protein S2 Monoclonal Antibody (1A9) (Invitrogen) (1:1000 dilution) or DYKDDDDK Tag Monoclonal Antibody (FG4R) (Invitrogen) (1:1000 dilution).



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    Measurements of SARS-CoV-2 concentration in supernatant of infected cells over time. (a) Results of 10 min iNTA measurements of samples with different time postinfection, as indicated. Teal and pink colors indicate different populations as extracted by the GMM. (b) Concentrations of particles as a function of time after infection for the two populations. (c) Upper: iSCAT contrast extracted from the iNTA data recorded for 24 hpi sample converted to backscattering cross section. Lower: C-iSCAT contrast converted to backscattering cross section. Colors indicate presence of fluorescence labels for <t>S-protein</t> (teal) and Tsg101 (magenta). (d) Exemplary C-iSCAT and fluorescence images for particles adsorbed on a cover glass. Arrows in the top panel indicate particles positive for S-protein (teal) or Tsg101 (magenta). Scale bar is 1 μm.
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    Measurements of SARS-CoV-2 concentration in supernatant of infected cells over time. (a) Results of 10 min iNTA measurements of samples with different time postinfection, as indicated. Teal and pink colors indicate different populations as extracted by the GMM. (b) Concentrations of particles as a function of time after infection for the two populations. (c) Upper: iSCAT contrast extracted from the iNTA data recorded for 24 hpi sample converted to backscattering cross section. Lower: C-iSCAT contrast converted to backscattering cross section. Colors indicate presence of fluorescence labels for <t>S-protein</t> (teal) and Tsg101 (magenta). (d) Exemplary C-iSCAT and fluorescence images for particles adsorbed on a cover glass. Arrows in the top panel indicate particles positive for S-protein (teal) or Tsg101 (magenta). Scale bar is 1 μm.
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    Measurements of SARS-CoV-2 concentration in supernatant of infected cells over time. (a) Results of 10 min iNTA measurements of samples with different time postinfection, as indicated. Teal and pink colors indicate different populations as extracted by the GMM. (b) Concentrations of particles as a function of time after infection for the two populations. (c) Upper: iSCAT contrast extracted from the iNTA data recorded for 24 hpi sample converted to backscattering cross section. Lower: C-iSCAT contrast converted to backscattering cross section. Colors indicate presence of fluorescence labels for <t>S-protein</t> (teal) and Tsg101 (magenta). (d) Exemplary C-iSCAT and fluorescence images for particles adsorbed on a cover glass. Arrows in the top panel indicate particles positive for S-protein (teal) or Tsg101 (magenta). Scale bar is 1 μm.
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    GeneTex monoclonal reagent s2 subunit spike protein, clone 1a9
    A Duodenal crypt with small acinus, and with intracytosolic and intranuclear inclusions with halo and apoptosis consistent with a viral infection (H&E 20 × magnification). B Positive immunohistochemical expression of the SARS-CoV-2 spike protein in these duodenal crypts (arrows, 20× magnification, COVID-19-S2-Subunit of the spike protein, clone <t>1A9).</t> C Immunohistochemistry showing that ACE2 is expressed in enterocytes with strong expression on the cell surface. In situ hybridization of antisense s-SARS-CoV-2 ( D ) and sense s-SARS-CoV-2 ( E ) confirms viral tropism and replication in duodenal biopsies. Viral RNA is visualized as red dots in the cytoplasm and nucleus of enterocytes. Blue staining represents nuclear DNA counterstaining of enterocytes with DAPI
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    Image Search Results


    Measurements of SARS-CoV-2 concentration in supernatant of infected cells over time. (a) Results of 10 min iNTA measurements of samples with different time postinfection, as indicated. Teal and pink colors indicate different populations as extracted by the GMM. (b) Concentrations of particles as a function of time after infection for the two populations. (c) Upper: iSCAT contrast extracted from the iNTA data recorded for 24 hpi sample converted to backscattering cross section. Lower: C-iSCAT contrast converted to backscattering cross section. Colors indicate presence of fluorescence labels for S-protein (teal) and Tsg101 (magenta). (d) Exemplary C-iSCAT and fluorescence images for particles adsorbed on a cover glass. Arrows in the top panel indicate particles positive for S-protein (teal) or Tsg101 (magenta). Scale bar is 1 μm.

    Journal: ACS Nano

    Article Title: Measuring Concentration of Nanoparticles in Polydisperse Mixtures Using Interferometric Nanoparticle Tracking Analysis

    doi: 10.1021/acsnano.4c04396

    Figure Lengend Snippet: Measurements of SARS-CoV-2 concentration in supernatant of infected cells over time. (a) Results of 10 min iNTA measurements of samples with different time postinfection, as indicated. Teal and pink colors indicate different populations as extracted by the GMM. (b) Concentrations of particles as a function of time after infection for the two populations. (c) Upper: iSCAT contrast extracted from the iNTA data recorded for 24 hpi sample converted to backscattering cross section. Lower: C-iSCAT contrast converted to backscattering cross section. Colors indicate presence of fluorescence labels for S-protein (teal) and Tsg101 (magenta). (d) Exemplary C-iSCAT and fluorescence images for particles adsorbed on a cover glass. Arrows in the top panel indicate particles positive for S-protein (teal) or Tsg101 (magenta). Scale bar is 1 μm.

    Article Snippet: For immunofluorescence measurements, 100 μL of inactivated SARS-CoV-2 samples were incubated 90 min at room temperature in glass-bottom dishes (ibidi), unbound particles removed, samples blocked with 4% BSA (w/v) in PBS and incubated overnight at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech) diluted 1:1000.

    Techniques: Concentration Assay, Infection, Fluorescence

    Journal: iScience

    Article Title: Identification and differential usage of a host metalloproteinase entry pathway by SARS-CoV-2 Delta and Omicron

    doi: 10.1016/j.isci.2022.105316

    Figure Lengend Snippet:

    Article Snippet: Mouse anti-SARS-CoV-1/SARS-CoV-2 Spike Protein S2 (1A9) , ThermoFisher Scientific , Cat#MA5-35946 RRID: AB_2866558.

    Techniques: Membrane, Virus, Recombinant, Modification, Expressing, Lysis, Transfection, Western Blot, Luciferase, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Construct, Software

    Journal: STAR Protocols

    Article Title: Protocol to isolate and assess spike protein cleavage in SARS-CoV-2 variants obtained from clinical COVID-19 samples

    doi: 10.1016/j.xpro.2022.101502

    Figure Lengend Snippet:

    Article Snippet: SARS-CoV-2 Spike Protein S2 mAb (1A9) (1:3000) , Thermo Fisher Scientific , Thermo Fisher Scientific Cat# MA5-35946, RRID: AB_2866558.

    Techniques: Expressing, Software, Reverse Transcription Polymerase Chain Reaction, Modification, Protease Inhibitor, Western Blot

    A Duodenal crypt with small acinus, and with intracytosolic and intranuclear inclusions with halo and apoptosis consistent with a viral infection (H&E 20 × magnification). B Positive immunohistochemical expression of the SARS-CoV-2 spike protein in these duodenal crypts (arrows, 20× magnification, COVID-19-S2-Subunit of the spike protein, clone 1A9). C Immunohistochemistry showing that ACE2 is expressed in enterocytes with strong expression on the cell surface. In situ hybridization of antisense s-SARS-CoV-2 ( D ) and sense s-SARS-CoV-2 ( E ) confirms viral tropism and replication in duodenal biopsies. Viral RNA is visualized as red dots in the cytoplasm and nucleus of enterocytes. Blue staining represents nuclear DNA counterstaining of enterocytes with DAPI

    Journal: Infection

    Article Title: Duodenal tropism of SARS-CoV-2 and clinical findings in critically ill COVID-19 patients

    doi: 10.1007/s15010-022-01769-z

    Figure Lengend Snippet: A Duodenal crypt with small acinus, and with intracytosolic and intranuclear inclusions with halo and apoptosis consistent with a viral infection (H&E 20 × magnification). B Positive immunohistochemical expression of the SARS-CoV-2 spike protein in these duodenal crypts (arrows, 20× magnification, COVID-19-S2-Subunit of the spike protein, clone 1A9). C Immunohistochemistry showing that ACE2 is expressed in enterocytes with strong expression on the cell surface. In situ hybridization of antisense s-SARS-CoV-2 ( D ) and sense s-SARS-CoV-2 ( E ) confirms viral tropism and replication in duodenal biopsies. Viral RNA is visualized as red dots in the cytoplasm and nucleus of enterocytes. Blue staining represents nuclear DNA counterstaining of enterocytes with DAPI

    Article Snippet: As previously described [ ], we focused on two established monoclonal reagents (Sinobiologicals to NP, clone 001, and Genetex to S2 subunit spike protein, clone 1A9).

    Techniques: Infection, Immunohistochemical staining, Expressing, Immunohistochemistry, In Situ Hybridization, Staining